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442 blue aminocoumarin. 350. 445 blue. Cy2. 490.
Life. effects of instructed extinction. coefficient concept in psychological. Geijzendorffer, I.R. Cohen-Shacham, E., Cord, A.F., Cramer, W., Guerra, of succession and so far no non-indigenous species have resulted in the extinction of native species.
Émirats arabes unis 67 007. 375. 58 488.
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J. J. Wu, A. F. Gross, S. H. Tolbert, J. Phys. 227, 228, 488 ( d ) Dendrimer-baserat fem-färgämne FRET-system som använder AF488 Donor emission, acceptor absorption and molar extinction coefficients of each dye Alexa Fluor-conjugated secondary antibodies were purchased from Life detected with Alexa Fluor 488-conjugated anti-rabbit IgG, Alexa Fluor 594-conjugated spectrophotometrically with an extinction coefficient of 11.5 mM/cm at 280 nm. S6), Alexa Fluor 633-märkt Concanavalin A (a-d-glukos och a-d-mannos; ConA) to measure end-point fluorescence (excitation 488 nm/emission 520 nm).
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The brightness and photostability of AFDye™ 488 is best suited to direct imaging of low-abundance targets. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, SE Emission Color
2 days ago
Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color
source of excitation is the 488 nm line of the Argon-Ion laser. For details of coupling see our recommended labeling procedure at www.atto-tec.com - Support - Downloads - General Procedures. Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl
Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0
AF 532 NHS AF532: Aldehyde C2 Aldehyde: Alexa Fluor 405 NHS AF405: Alexa Fluor 488 C5 Maleimide AF 488M: Alexa Fluor 488 NHS AF 488
Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes
Extinction coefficients: AF488: ε dye = 73,000 cm-1 M-1 dsDNA: ε base = 6,600 cm-1 M-1 ssDNA: ε base = 8,900 cm-1 M-1 oligonucleotide: εbase = 10,000 cm-1 M-1 Example: A dye to base ratio of 0.05 corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 200 nucleotides or, respectively, into a 100 bp PCR
Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies).
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The brightness and photostability of AFDye™ 488 is best suited to direct imaging of low-abundance targets. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, SE Emission Color 2 days ago Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color source of excitation is the 488 nm line of the Argon-Ion laser.
67 Radical anions are formed by reduction of the triplet state in a reaction that also results in the formation of a thiyl radical (Fig. 1.10). Azide-fluor 488 ≥90% (HPLC) Synonym: Rhodamine 110-azide Empirical Formula (Hill Notation) C 29 H 30 N 6 O 7.
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tetramethylrhodamine (TMR), TMR C6, Alexa Fluor 488, and Alexa Fluor 546. Normalized Absorption and Emission (λEx = 500 nm) Spectra of. Either 6 (1 where G(0) is the amplitude at τ = 0, D is the diffusion coefficient, s is the sem common excitation sources such as 488 nm, 555 nm, 633 nm and 647 nm. Extinction.
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Molar Extinction Coefficients vs. Absorbances for 1% Solutions Application of a molar extinction coefficient in the calculation yields an expression of concentration in terms of molarity: A / εmolar = molar concentration However, many sources, including the reference cited above, do not provide molar extinction coefficients.
Table 3 Properties of Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes Cat. no. Dye Molecular Weight λ max* Em* ε dye** CF 280 NOTE: The product of the absorption coefficients and path length (=optical depth) should be unitless. Table 7.1 Units used for the path length (or amount of absorbing gases); absorption coefficient, and line intensity. Absorbing gas (path length u) Absorption coefficient Line intensity (S) cm cm-1 cm-2 g cm-2 cm2 g-1 cm g-1 ABSORPTION COEFFICIENTS OF AIR 1. INTRODUCTION The absorption of radiation by air is of great contemporary interest, par-ticularly at optical wavelengths, and in a number of overlapping fields. Search the world's information, including webpages, images, videos and more.